Comparing the conductivity of two different solutions

Conductivity is perhaps the most misunderstood and erroneously used measurement in hydroponic culture. This has a lot to do with conductivity also being called a “totally dissolved solid” (TDS) measurement and the conductivity scale being expressed in “ppm” units, concentration units which only cause confusion in this area. Today I want to talk about an important consequence of this confusion that happens when you try to compare the conductivity of different nutrient solutions. I’ll talk about a recent case I encountered and how it generated significant problems due to a natural misunderstanding of how conductivity works.

A grower wanted to run a side by side trial of two nutrient formulations using identical growing conditions. This grower then decided that the best way to do this was to ensure that the conductivity and pH of the two solutions were identical after preparing the nutrient solutions, then they would both be equivalent in terms of their strength and differences in results would be entirely due to the differences in ionic ratios between both of them. The media was the same, the environment was the same and plant genetics were the same.

However there was a small problem with this thinking. The same conductivity across two different solutions is not the same thing. You might think that using a conductivity of 2.0 mS/cm across two different nutrient solutions might mean that their “strength” is the same, but in reality the strength of a solution – as per what a plant really experiences – is determined by its osmotic pressure and osmotic pressure – although proportional to conductivity within the same solution – cannot be extrapolated when the composition of the solution changes. This confusion is further expanded when people see the conductivity numbers in ppm because the expression in mg/L makes them think there is the same “amount of stuff” in the two solutions. This is not the case.

All the ppm does is tell you that your solution has the same conductivity as a reference with that ppm concentration (commonly NaCl or KCl) but it tells you nothing about how many dissolved solids are really present within your nutrient solution. Given that non-conductive substances also affect the osmotic pressure of a solution it can happen that a nutrient solution with the same conductivity as another one in reality has a lot more dissolved solids, making it far more concentrated in real terms compared to the other one.

In the above mentioned particular case one solution had a chelating agent that effectively made a significant number of ions neutral in charge (effectively making them non-conductive) reducing the measured conductivity by around 20% at the same osmotic pressure as the other solution. So while the grower was feeding the two solutions at the exact same conductivity, the second solution was around 20% more concentrated in real terms – osmotic pressure terms – compared to the other one. Plants responded very negatively to this – as the conductivity was already quite high – so the grower erroneously assumed that this was due to the ionic ratios instead of it simply being due to an error in judging concentrations. The second solution was a lot stronger in real terms, although the conductivity was the same.

When comparing two nutrient solutions you should therefore resort to measurements different than conductivity because the conductivity of two different solutions with different ion compositions cannot be compared, the same level of conductivity will result in two completely different osmotic pressure values. Their strengths will not be the same. If you want to compare two different solutions at the same real strength then you need to use an osmometer to determine this point and sadly osmometers are neither cheap nor practical to use.

However another possibility is to simply compare at a constant concentration of a given element. Have a lab analysis of the two fertilizers made – remember you cannot trust labels to give you the real composition values – calculate how much of a given element, for example N, is present at a given application rate and then dial in the other fertilizer to match that N concentration. The osmotic pressures will probably be different but at least under this sort of A/B test you will be comparing apples to apples in the sense that the only variable will be the N:X ionic ratios between the two solutions. Total strengths will differ but this will be due to differences in ionic ratios, which is probably what you want to test.

 




Controlling aphids in a hydroponic crop. Part 1.

Without a doubt aphids are one of the most common pests affecting crops worldwide. There are both root and leaf aphids, the former which generally live only around plant roots – producing winged offspring only to infest new plants – while the later live generally in plant stems, leaves and – when infestations are bad enough – even within plant flowers and fruits. Today we are going to talk about several alternatives to deal with aphids, from traditional insecticides to more natural alternatives such as biocontrol options. We are not going to discuss mechanical options here – we’ll leave that to part two – as we’ll focus only on chemical and biological control within this first part.

There is one clear winner when controlling aphids. At the present time nothing will beat neonicotinoids in fighting aphids as these insecticides are very effective against a wide range of sucking insects (which are insects that suck material out of plant tissues). Originally made during the mid 1980’s and massively popularized during the 1990’s (see here) insecticides like imidacloprid have been huge winners in the fight against aphids. They are applied via soil applications – no need for foliar applications – where they are absorbed by the roots and effectively make plant tissue completely toxic for aphids, affecting their nervous system.

However everything is not rosy with insecticides like imidacloprid. Neonicotinoids affect beneficial insect populations – bees in particular (see here) – so they are not good for the environment in general. As a secondary problem they also remain within plants for a really long time so they should only be used when plants are a significant time away from harvest (at least 60 days is usually recommended). When using on edible crops make sure you get a formulation that has been specifically designed for this purpose (like this one). However some legislations require no imidacloprid to be present in plant tissue meant for human consumption so it is important to check with regulatory guidelines regarding its use. There are several studies showing how imidacloprid can accumulate in fruits and flowers (see here for an example in maize, here for an example in tomatoes).

Perhaps we can resort to less damaging alternatives but still control aphids effectively. Predatory insect applications don’t work very well (another post about this coming soon!). But one of the best alternatives I have found so far is to use Lecanicillium Lecanii – and other Lecanicillium species – as a parasitic fungus to attach the aphids. Not only are they effective in attacking aphids but they can also be used as a two-for-one control against powdery mildew and other pathogens as well (see herehere and here). I have had a few recent experiences with customers that have had good success using such fungi to control aphids in several crop types, including parsley and tomatoes. I have had great personal success in parsley, basil and mint plants. These two are the products that I have seen used containing this fungi (here and here). Image below taken from this paper and first image in this post taken from this paper.

There are also some naturally occurring insecticides that can be used, such as neem oil based products. The problem with these insecticides is that they do work – sort of- depending on the plant and aphid specie you are trying to tackle (see here). Generally 0.2-0.5% emulsions of the oil are effective against aphid populations with such application generally killing most aphids when they work (see here and here). Although neem oil applications shouldn’t be considered as a stand-alone solution they can provide a strong head-start when dealing with aphid infestations since they can kill a large portion of the population – if they are susceptible – without harming beneficial insects that might be predating on the aphids already. Last image in this post taken from this paper.

For root aphids the option to use beneficial nematodes also exists. These worms enter the insect bodies and feed on their internal fluids, killing them in the process. However in contrast with fungal spores nematodes do actively seek their pray, so they will hunt the aphids down within the media while a fungal spore needs to meet the aphids randomly. Single nematode species like Heterorhabditis bacteriophora can attack aphids although combinations using other nematode species are usually more effective since different nematodes usually attack different species with different efficiencies (see here). Mortality rates when using nematodes are usually at most around 80% so they need to be effectively used in combination with other methods to provide effective control.

As you can see there are several options for aphid control in your crops. Although using synthetic insecticides like imidiacloprid might be the most effective alternative there are in fact other options that can also be used successfully if the use of a neonicotinoid is not desired. Application of Lecanicillium species has shown to be most effective in peer reviewed studies while nematode and neem applications can help compliment this approach and provide a defense against other insects and pathogens. On the next post in this series we’ll talk a bit more about additional aphid control using mechanical means that are neither chemical nor biological.

 




Five ways to increase your seed germination rates

When you start plants from seeds one of the most important things you want to achieve is a very high and fast germination rate. However if you try to do seed germination without any additional effort you will most likely reach sub-optimal results since there are some natural factors that hinder seed germination that need to be eliminated in order to achieve the best possible results. Today we are going to talk about five things you can do in order to provide the best conditions for the germination of your seeds.

Temperature is very important. When doing seed germination one of the most critical factors is seed temperature. Some plants require cold temperatures to germinate – for example spinach’s germination rate drops to about half when you go from 15 to 25°C – while other plants require higher temperature – for coriander it’s basically the opposite. For your seedling emergence rate to be as high as possible ensure that you are giving them the temperature they ideally want, which depends on the plant species. Below you can see a table with germination temperatures for several plant species.

Pretreat seeds with PEG-6000. Polyethylene glycol treatments can dramatically increase seed germination rates (see here). We have known this since the mid 1970’s and we have also known that the optimum treatment duration and air-drying effects change according to plant species. Applying a general PEG-6000 treatment, as a I described here a few years ago, might or might not work depending on the plant you’re trying to work with. For best results you need to search the scientific literature for the best PEG-6000 treatment or – if this information is not present – design your own experiments to figure this out.

Seed disinfection. Seeds are usually covered in microorganisms that can seriously impair seed germination rates. In order to eliminate this issue seeds need to be disinfected prior to germination with a chemical agent (most commonly either hydrogen peroxide or sodium hypochlorite solutions). For this purpose solutions in the order of 0.1-2% NaClO are generally used with different soaking times varying between different papers. You can read more about this sort of process here. Treatments are usually quick with disinfection lasting only a few minutes with subsequent plain water baths to eliminate any excess oxidant.

Introduce some good guys. In the same way that there are pathogens that can hinder seed germination there are some “good guys” that can stimulate seed germination. In particular there are trichoderma species that have been known to increase germination rates for some plant species. For example in this paper using okra (see here) there was an important increase in germination rates when using T. harzianum as a beneficial fungi. You should look for some scientific literature surrounding the species that interest you or – if that’s not available – apply a product that contains a few trichoderma species.

Using GA3 for stimulation. The final trick in your arsenal to increase germination rates is to use Gibberellic acid to stimulate your seed germination. Optimum concentration of gibberelic acid, treatment lengths and effects depend fundamentally on the plant species used but this is acknowledged to be a quite universal stimulant for seed germination rates in the general scientific literature. You can read this paper to see the effects of gibberelic acid on a wide variety of species found in western Australia (so that you can grasp how different its action can be). For particular species you can find articles like this one – for tomatoes – where different GA3 concentrations are tested to figure out the best application rate. The effect can be quite dramatic as in the image below (taken from this paper).

In the end there are many things we can do to improve seed germination and the above is by no means an exhaustive list. For particular plant species there can be other tricks – for example things like scarification – which can lead to important improvements in germination rates as well. However the above advice is quite general and can probably help you increase germination rates for a wide variety of plant species.




Making your own DIY plant rooting gel

Cloning is a very common technique used by a large variety of plant growers. When growing plants from seeds there is an important unpredictability factor in what you might get so cloning ensures that you get a clear genetic copy of the parent and therefore removes a lot of the variability inherent to the growing process when starting from seed. To perform the cloning process most growers use the aid of rooting hormones which are usually sold in the form of a gel at high prices. Today we are going to learn how to make our own DIY plant rooting gel using ingredients that can be easily bought online for a fraction of the cost.

Rooting gels have basically four ingredients. A rooting hormone (active ingredient), a gelling agent (usually an acrylic acid polymer), a base (needed to increase the viscosity given by the gelling agent) and a preservative (because fungi eat anything). Today we are going to talk about making a rooting gel without any preservative – which is simpler – so don’t make very large quantities because it can spoil after some time (probably will last for a month or so). To make this you will need the following:

  • Distilled or RO water
  • Indole-3-butyric acid (you can get it here)  0.69 USD/g
  • Carbopol 940 (you can get it here) 0.09 USD/g
  • Potassium hydroxide (you can get it here) 0.02 USD/g
  • Two containers for mixing (one around 60% of the volume you want to make, the other around 120%)
  • A scale that can weight with enough precision according to the amount you want to prepare (for 1L you will need a +/- 0.1g scale).

Warning: Potassium hydroxide is a very strong base. Handle with a lot of care wearing protective eye wear and nitrile or PVC gloves. Do not agitate it before opening it since KOH powder is very caustic.

Once you get these ingredients the process is quite simple. For a one liter preparation add 500 mL of water to one container (we will call this one A) and 500mL to another container (which we will call B). Add 3.0g of the Indole-3-butyric acid to the A container along with 0.6g of potassium hydroxide and mix until both are dissolved. Heat the water in container B to 120-140F (48-60°C), stop heating and add 9.0g of Carbopol 940. Mix the water in container B thoroughly, the Carbopol 940 might take a long time to get hydrated and get into solution, stir it until there are no visible clumps (this can take around 15-60 minutes).

Once this process is done wait for B to cool to ambient temperature, then mix A and B slowly (in whichever has the largest container). When you do this the viscosity of the mixture will start to increase exponentially and you will have your rooting gel preparation. The amount of money it takes to prepare 1L is around 3 USD while the most popular rooting gel products online are charging you around 16 USD for 100mL of basically the same thing. This means that you will be saving 98% of your rooting gel costs if you make your own.

There are some other additives – including preservatives and biostimulants – that we could add to make a better product, but that’s a topic for another blog post.




Building your own DIY high power LED lamp: Part One

It is no mystery that LED technology has evolved greatly during the past several years. We are now up to the point where anyone can buy LED lamps to replace HPS fixtures, with full spectrum LED configurations that have showed to be just as good – or sometimes even better – at growing crops (see here for a post about LED lights Vs HPS). However these lamps are often very expensive – most commonly around thousands of dollars to adequately replace a 1000W HPS. Within these series of posts I am going to talk about how you can build your own LED lighting to replace HPS lights for pennies on the dollar compared to commercial LED fixtures.

WARNING: Mains voltages (110-220V) can be extremely dangerous. Please make sure that you know what you’re doing if you’re going to follow these instructions. All of this information is provided “as-is” with educational purposes only. Make sure you follow all safety precautions when working on mains electricity. 

There are several ways in which you could build your own LED lamps. This usually involves building an aluminium case with fans, putting an LED driver inside and then using that driver to power rows of different light emitting diodes. A driver is basically a transformer not unlike a computer PSU that takes voltage from the mains and dials it back down to a lower voltage that you can use across a row of diodes. Most commonly commercial lamps use combinations of 3W diodes with narrow focusing elements with sometimes higher wattage elements with wider focusing elements. Building a configuration like this can be done but it is a laborious that we can avoid using some of the latest advances in LED technology.

To make a simple high power LED lamp we should absolutely forget about putting together LED elements of different colors. This involves a lot of wiring and makes the lamp fundamentally more expensive. To replace them we can use white diodes instead which although far less efficient – as they are basically blue diodes whose light is absorbed and re-emitted by a phosphor – can give us all the different colors we need in the proportions we need them. The image above shows you the spectrum of different white diodes, as you can see we don’t want the 5000-8000K or 3700-5000K LEDs – which emit a lot of blue light we don’t need – but we need the much “warmer” 2600-3700K diodes which produce a lot of light in the red region of the spectra, with enough blue to provide us with close to a 1:3 ratio. Although this light spectra is still not ideal compared to what plants absorb it will easily able to replace a 1000W HPS.

To make things very simple and avoid using a separate driver we can use 150W LED cobs that include their own driver and are fed directly with 120/240V electricity (like the ones here). As I mentioned we want the lower temperature spectra white diodes so go for the “Warm white” and make sure the temperature description says it is at least 3200K or lower (if you’re looking at another source). The publication above contains 150W cobs that can do 2500-3200K so they can be considered ideal for this application. For every 150W cob you install you should also install a 2A AC fuse for that cob only to ensure that if anything bad happens the power will be cut almost instantly. Since these cobs are wired directly to mains electricity you should be specially careful with having proper safety precautions (proper soldering of the wires, solders protected with isolating material (like silicon) fuses for each cob, etc). 

Of course the cobs are only half the setup. We need to place these cobs on top of an appropriate heatsink and then also ensure we have fans for it. You can buy a properly sized aluminium heat sink here. Since cobs measure 16×40 we can comfortably glue two cobs to the bottom of a heat sink of profile A (146x22mm) with a length of 400mm. To glue the cobs to the heatsink you should use proper arctic silver thermal adhesive (you can find it here). For fans you can place 2 12cm Fans on top of the above. There are several fans that work with 120-240AC that you can use, for example these fans work with 120V. This setup will give us a 300W LED lamp, with 2 fans that should be able to keep the heatsink temperatures in check. All of this for a total of around 83 USD, let’s call it 100 USD after adding fuses, cable and other parts you might require.

The above lamp will not replace a 1000W HPS on its own, for this you will need at least 4 cobs – meaning two of the above lamps – which should give you 600W of LED power that should be close to the PAR of a 1000W HPS light. This for the cost of only 200 USD (far less than the commercial LED replacement lights). I am in the process of making my own so I will be able to give you some additional details as soon as I get the parts and finish building my own setup. In part No.2 of this series of posts I’ll show you the results of my work and what it does in terms of photon flux and PAR.




What is the ideal nutrient solution temperature in hydroponics?

One of the simplest variables that can make a substantial difference in crop yields in hydroponics is the temperature of the nutrient solution. Nutrient absorption by plants is mainly controlled by chemical processes within their roots and the efficacy of these processes is determined in an important part by the temperature the roots are subjected to. Since plants don’t have a mechanism for active temperature regulation they just react to changes in temperature in order to best adapt to the environment that surrounds them. Today I will be talking about the optimum solution temperature in hydroponics, what influences this value and what factors we must consider when deciding what temperature to use in our hydroponic system.

Solution temperature affects several important variables. Oxygen solubility changes as a function of temperature – decreasing as temperature increases – so as you increase the temperature the availability of oxygen to plant roots starts decreasing. As you increase temperature however the speed of the chemical reactions in plant roots increases, so there is an increase in respiration rates as temperature increases. The ideal temperature is therefore always a compromise between this decrease in oxygen availability and the increase in metabolic rate that is given by higher temperatures. For almost all commercially grown plant species optimum solution temperatures will be in the 15-30°C (59-86F) range due to this reason.

However there is no rule of thumb for optimum solution temperature selection in hydroponics. It should be clear that since different plants evolved across different conditions some of them perform better at lower temperatures and some others do better at higher temperatures. We know for example that the optimum nutrient solution temperature for potatoes is in the 20-25°C range (see here) while the optimum temperature for plants like cucumbers is higher, at 28°C (see here). For some plants like onions the best solution temperature can actually be a bit higher, even in the 26-30°C range (see here). Others like lettuce and baby leaf crops actually prefer much lower temperatures, with optimum results near 20°C (see here and here).

It is then clear that picking a random number between 15-30°C is not enough, a careful study of the plant specie being grown has to be carried out in order to select an adequate temperature. It is also important to note that higher temperature choices do not come without problems. We know for example that pythium and other infections are associated with increases in temperature since pathogen metabolism is also enhanced under warmer conditions (see here and here). This shows how even though the optimum temperature for tropical flowering plants is usually in the 25-30°C range, it is usually not common to see optimum results at these temperatures due to the potentially higher prevalence of diseases. This is most probably why growers usually go with a lower temperature in the 20-25°C to avoid risking diseases at a higher temperature.

If you want to try higher temperatures it is therefore better to go with sterile type hydroponic systems where microbes don’t play an important role and to implement measures – such as silicate additions to the nutrient solution, UV filtration and constant oxygenation – to ensure that disease prevalence is as low as possible. Also avoid adding any source of organic carbon (like sugars) as these can play an important role in feeding incoming pathogens. Big gains can be obtained with a better solution temperature control, provided that diseases are controlled and a temperature adequate for the plant being grown is selected.

 




Are High Pressure Sodium (HPS) Lamps better than LEDs?

Growers who use artificial lighting usually prefer high pressure sodium (HPS) lamps to do the job. Not only do HPS lamps have a very high photon flux but compared to metal halide (MH) lamps they have a much more prominent red spectral component and therefore a significantly larger dose of photosynthetically active radiation (PAR) per watt. However during recent years light emitting diode (LED) lamps have become much more efficient and have started to compete for the artificial lighting domain. However is there any advantage to using LED lights over HPS lamps? Are HPS lamps always going to be the winners? Today we are going to look at the science comparing HPS and LED lamps to see if there is currently a winner between the two.

The above graph shows you the PAR spectra. Basically this tells you which wavelengths of light are most prominently absorbed by plants. From this diagram it is clear that plants have peak absorptions around the blue and red parts of the spectra while the green section of the spectra is absorbed much less intensely and instead reflected (the reason why most plants look green). Ideally we would want lamps that have peaks in the regions of the spectra where the PAR peaks as well and we would like to have the highest peak in the red which is the region where we get the most efficient photons for the photosynthesis process.

In HPS lamps our spectra is basically fixed by the nature of the light source while in LED lamps we can tune the light source a lot. This is one of the reasons why there is such confusion when comparing HPS and LED lamps. Since LED lamps can be tuned so much it isn’t surprising that there are a large variety of cases where growers have experienced worse results from LED lamps compared with their HPS counterparts. With HPS lamps you basically buy one 1000 W lamp and you’re done while with LED lamps things such as the color distribution of the diodes being used and the focusing elements they have installed can make a tremendous different.

Checkout this study comparing LED and HPS lights to grow lettuce and radishes. The picture above shows you the results they had with HPS lamps compared with 3 different experiments using different LED distributions. A person running setups 2 or 4 would have thought that LEDs are worse than HPS lights while someone using setup 1 would have concluded that LED lamps are simply much better. This is why some growers will tell you that LED lamps are the greatest thing on earth while others will tell you they are never as good as HPS — they simply have used different lamps. Notice that in setup 3 a complete breakdown of the photosynthetic process happened.

In the above experiment growers used 4 LED types, 455nm, 640nm, 660nm and 735nm LEDs in a roughly 10:120:10:1 ratio. In setup 2 the 640nm LED intensity was reduced by a factor of 1.5, in the setup 3 the 735nm component was changed to nighttime only and in setup 4 the 735nm LED was changed to only two hours during nighttime. You can see how the decision to change a light source that contributed less than 2% of the total light flux to nighttime had a very important effect. This is because the 735nm wavelength has a circadian rhythm effect that can substantially change how the plant responds. Just turning on 2% of the LEDs at the wrong time completely turned around the results.

With the above it is not surprising that we find contradictory evidence in the scientific literature. Articles like this paper on cucumbers find that HPS provides better growing efficiency compared to LED lamps in line with other articles like this one on lettuce. However we should bear in mind that the LED lamps used are always different and the fact that a LED array provides worse results compared to HPS does not mean that this is true for all LED lamps overall. Since LED lamps can be tuned so much it is almost certain that for a given plant specie you will always find an LED combination that gives you at least the same results as an HPS lamp.

Nonetheless the power savings from LED lamps also need to be considered. In experiments where comparable photon fluxes are used LED lamps tend to provide savings of at least 30-40% in terms of power consumed from the lamps only while these savings can reach even higher values when considering the additional cooling needs of HPS lamps (that are often much lower for LED lamps).

Per the above LED lamps are definitely worth considering as a replacement for HPS lamps. However you need to properly build your LED lamps such that the photon flux and spectral composition does provide you with results that can surpass those of regular HPS. Building a lamp that is underpowered or that has an inappropriate spectral composition can indeed cause you to get results inferior to those of HPS lamps. This is most probably the reason why so many growers are so reluctant to move to this type of solutions when using either only artificial or supplemental artificial lighting.




Five dos and don’ts for automated pH control in hydroponics

The pH is one of the most important variables to control in hydroponic culture as it plays a key role in the availability of different ions and their absorption dynamics. Although most growers control pH manually it is often desirable to implement automatic pH control so that you can ensure that your solution always stays within an optimum range. This is especially true in recirculating systems where correcting the pH of the solution after it goes through the plants’ roots is necessary. Today I want to share with you five dos and don’ts when implementing automated pH control in hydroponic culture.

Do test your pH meter frequently with a buffer solution.  In hydroponics pH meters can lose calibration rather quickly as a consequence of being immersed in a nutrient solution that is at a lower ionic concentration than what’s ideal for most glass electrodes. This means that testing your pH meter with a buffer solution often – every week is ideal – is necessary in order to ensure that you are getting accurate readings. If the reading is not accurate you can then recalibrate the meter.

Do recondition your pH meter every month. In line with the above and in other to increase the life of a pH meter and each calibration it’s necessary to immerse the pH electrodes in a pH 4 or 7 buffer solution every month – for at least 2-3 hours – to ensure that the ionic content of the electrode is restored and the glass membrane’s responsiveness remains accurate. If you do this your electrode will be happier and you will need to calibrate less frequently. If an electrode is covered in biofilm the putting it in a hot bleach solution for half an hour before the buffer immersion is also necessary.

Do use electrodes designed for constant immersion. Regular pH electrodes – including those sold with some automated pH controllers – are not meant to be immersed the whole time and therefore get damaged and lose calibration much more quickly when used in this manner. To get best results use pH electrodes that are fabricated with long term immersion in mind. I wrote a blog post about these electrodes and why they are different than traditional pH electrodes.

Do place your electrode as far as possible from your pH changing inputs. When using a pH controller you should place the pH probe as far away as possible from the place where your pH up/down solutions will enter the hydroponic system. This is so that your pH electrode can get a slow change in pH as the pH up/down is mixed with the entire reservoir. Placing the probe close to the inputs will cause very erratic changes in the pH that do not really reflect the effect of the addition across the entire reservoir.

Do have addition limits in your controllers. Allowing a pH controller to add as much substance as needed to correct the pH can be a very bad thing to do. This is because several things can go wrong – pH probe losing calibration, controller getting damaged, electrical noise etc- that can cause unnecessary levels of addition that can kill an entire crop. Always have controllers where maximum additions per unit of time can be specified so that the possibility of this happening is minimized.

Don’t rely on a single pH probe. Although single probe controllers are the most common they can also be the most dangerous. A pH probe can get damaged, it can lose calibration or it can give erratic readings due to other reasons (for example electrical interference from other things in the reservoir). Therefore it’s always best to use two-probe controllers where readings are always verified across the two probes to ensure that the reading the controller is getting is accurate. If you have a commercial enterprise then this is a must, you wouldn’t want to lose an entire crop due to a bad pH probe adding a ton of acid/base to your solution.

Don’t aim for a specific pH value. A pH controller should not aim for a specific value of pH but to maintain pH within an adequate range. Usually the best way for a controller to act is to have a range with high/low thresholds where the controller will act to take the pH to the middle of the range when these thresholds are exceeded. For example a controller can be told to maintain pH in the 5.6-6.4 region and then it will act whenever the pH reaches 5.6 or 6.4 to take it back to 6 when any one of these two thresholds is breached. However if the pH is at 6.4 and the controller drops it to 5.8 it will not try to then bring the pH up (because it’s above the lower threshold).

Don’t place your pH probes near pumps or other electrical equipment in reservoirs. A pH probe takes an electrical measurement and is therefore prone to electrical interference. Having a pH probe close to other electrical equipment – especially those that draw significant current – can cause those wires to induce currents in the pH probe wires and generate all sorts of issues with pH readings. Always place pH probes away from pumps and ensure the pH probe and pump wires are never tangled together.

Don’t use very concentrated acid/base input solutions. A pH controller will be doing very fine control over a small pH range so it won’t need a very large amount of acid/base to do this job. Using very concentrated acid/base can cause the pH controller to completely overshoot its targets and cause it to either cause the system to get into an undesirable state – for example a very low pH if the controller can only add acid – or enter a loop where acid additions are followed by base additions in an endless cycle. Usually you want your acid/base mixtures to be concentrated enough to shift the pH over their addition volume but not much more than that. Strong acids/bases in the 10-20% concentration range are usually more than enough for this job.

Don’t ignore your controller’s data. A pH controller will do its job – control pH within a range – but it will not tell you whether your system is doing ok or not from a plant-health perspective. How often your pH controller has to add acid/base and how much acid/base it’s using to perform its job are important pieces of information that you need to take into account in order to ensure that your system is working properly. Remember that pH controlling substances often also contribute nutrients – like phosphorous or potassium – so it’s important to keep all these additions in check.

Of course pH control is no simple task and different pH controllers will have different advantages and disadvantages. However doing what you can to ensure proper maintenance – cleaning, conditioning electrodes, having proper placement, etc – can go a long way in ensuring that your setup behaves as ideally as possible. If you can then I would advice you build your own controllers using things like arduinos, raspberry pi computers and robust immersion pH probes so that you can have an optimum setup that can deliver all the advantages of pH control with as few disadvantages as possible. I’ll write about building your own pH controller in a future post.

 




Using triacontanol to increase yields in hydroponics

Usually additives used in hydroponics need to be added in rather large quantities to obtain palpable results. Molecules like salicylic acid – which we have discussed before – need to be used in concentrations in the order of 10-4 to 10-2 M to obtain a significant effect. This means that you need to use quantities in the order of 20-150ppm of most additives in order to see a significant result. However there is a molecule called 1-triacontanol that can generate very significant results with only a fraction of that concentration. Today we will talk about this substance, what it does, how to use it and why it’s such a desirable tool in your hydroponic additive arsenal. Many of the things I will talk about in this article are derived from this 2011 review on triacontanol (make sure you read that for a deeper insight into why this molecule works).

Triacontanol is a very long fatty alcohol. Each molecule has 30 carbon atoms linked in a linear structure which makes this molecule extremely hydrophobic and hence very hard to dissolve in something like water. Using triacontanol therefore involves dissolving this molecule in something other than water –  for example Tween 20, chloroform, methanol – before adding water in order to prepare an emulsion for use in either root applications or foliar feeding. Most research using triacontanol has used foliar feeding as this is the easiest way to control the application of the molecule and also how it seems to have the largest effect.

The effects of this molecule are not short of miraculous. Triacontanol is usually applied in concentrations on the order of 10-7 to 10-9 M, which means it is used from around 0.01 to 1 ppm. This means that we use about 1000 times less triacontanol than other additives in order to obtain a meaningful result. The table below shows some of the effects that triacontanol has showed in peer reviewed studies, with plant height, weight and yields increasing across a variety of different species, from tomatoes to japanese mint. Papers on other plants besides those on the chart have also been published, for example triacontanol has showed to significantly increase yields in lettuce crops (here).  Some studies have also found that the effect of triacontanol can also be enhanced through the use of magnesium or in conjunction with other hormones (here).

With such an impressive array of effects and such a low expected toxicity – due to its very low solubility – it’s definitely one of the best additives to use to get production gains in hydroponic crops. This also makes it one of the most commonly used substances in commercially available grow enhancers. Nonetheless since it’s used in such a small quantity it’s very easy for someone to buy a small amount of triacontanol and use it for years before running out. You can buy small amounts of triacontanol as a powder (there are several reputable sellers on ebay) and you can then prepare your own concentrated triacontanol solution in Tween 20 – not water – that will last you for ages. A liter of 2000ppm solution of triacontanol will last you for 1000-2000 liters of foliar spray. You cannot get more economical than that.

The optimum application rate and frequency for triacontanol varies across different species but if you want to take an initial guess use a foliar application of a 0.5 ppm solution every week. There is usually a sweet spot for concentration – after that you start to see a decrease in results compared to the highest point – so you want to start below a 1 ppm application rate. For some crops repeated applications might be unnecessary – with just one or two applications giving most of the effect through the entire crop cycle – while for others you do want to apply every week. How you initially dissolve the triacontanol to make your concentrated solution is also important with Tween 20 being the most ecologically friendly – although not the easiest – option.




Salicylic acid and its positive effect in hydroponics

When looking for ways to increase crop yields we usually want something that is safe for the environment, safe for us and able to give us a substantial bang for our buck. From the multitude of additives that have been researched during the past 30 years one simple organic molecule seems to fit all the requirements very well: salicylic acid. Today we are going to talk about why this additive is so interesting for use in hydroponic culture, the results it has shown in peer reviewed publications and how we can use it to increase our crop yields. For those of you interested in this molecule I would also recommend reading this 2010 review which contains a much more detailed look into the scientific literature surrounding salicylic acid research in higher plants.

Salicylic acid is a simple organic molecule with the structure showed above. We have known for a long time that plants produce it and we knew almost right from the start that it played a key role in plants’ response to diseases and stress (see here for some early insights from Tobacco cultivation). Salicylic acid is used as a signaling molecule in plants (a.k.a hormone), moving from stressed organs to non-stressed ones as the plant is attacked. However its role is much more complex, having functions related with chloroplast creation, inhibition of fruit ripening and many other important processes.

After learning that this was an interesting molecule it wasn’t long before people started studying whether exogenous applications provided any benefit. We have learned that it enhances dry mass and leaf area in corn and soybean (here), that it can enhance germination in wheat (here), the oil content in basil (here), the carbohydrate content in maize, etc. There are also several studies pointing to improvement in root development – even from foliar applications (here) – suggesting that this hormone is able to increase plant productivity through several different mechanisms. The incidence of diseases can also be reduced dramatically by salicylic acid applications (here).

We also know this molecule has important effects on the flowering process. It can induce earlier flowering in plants and can often cause larger fruit settings in some plants (like papaya (here)). Most importantly foliar spraying of tomato and cucumber plants with salicylic acid has showed important increases in yields (here). It is therefore clear that exogenous applications of salicylic acid can have many important benefits in crop production and this is therefore an important candidate to consider for enhancing crop production.

But how do we apply it? Most commonly this molecule is applied in foliar feeding regimes, although in some cases it is also applied directly in hydroponic solutions. Most commonly concentrations in the order of 10-5 -10-4 M are used since it has been showed across a few studies that negative effects start to show up when the concentration level reaches 1mM. This means that regular doses will be around 1-100 ppm with the lowest spectrum of dosage being preferred if the effect on the particular plant is unknown. The solubility of salicylic acid is 2.48g/L at 25°C so concentrated solutions of up to around 20-30x can be prepared without issues to make it easier to apply on plants. The preparation of more concentrated solutions requires some tricks but it certainly can be done.

Salicylic acid also has the advantage of being a very safe molecule so it can be applied without a lot of worry in order to experiment with its effects. For testing on new plants foliar applications of 20-30ppm would be most common, with applications usually carried out once every 5-10 days. The frequency of application as well as the best concentration to use will of course depend on the particular plant you’re growing. There are also several other molecules that can be used with salicylic acid to enhance its effect on some plants, but this will be the focus of a future post.

Finally it is also worth noting that salicylic acid is not aspirin (aspirin is acetylsalicylic acid, a related yet different molecule) so if you want to experiment with this additive you should buy salicylic acid instead of just “dumping some aspirin” into your foliar or hydroponic nutrient solution.