Kinetin was the first cytokinin ever discovered. Scientists have used it extensively to stimulate cell division in tissue culture, as it is a powerful growth hormone. However, there isn’t a clear understanding of the effects of kinetin in large flowering plants, reason why it hasn’t been widely used as an additive in plant culture. In this post, we are going to take a look into the practical application of kinetin. We are going to look into published research and discuss whether kinetin could be used to enhance plant yields. I will refrain from discussing the history and chemical structure of kinetin, for a basic introduction about kinetin and its history, I suggest reading this paper (1). I will also use some information contained in this review (5).

The effects of exogenous kinetin

In tissue culture, what kinetin does seems to be clear, it promotes cell division in the presence of auxins. However, for large plants in soilless media, the effect does not seem to be that straightforward. One of the first thorough studies of kinetin in flowering plants was done in the early 1960s (2). In this study, tomatoes, cucumbers, and peats were grown in solutions containing different concentrations of kinetin, going from 10^-5 to 10^-7 molar. The researchers showed that kinetin in solution behaved like a gibberellin inhibitor, directly suppressing plant height as a function of concentration. The plants developed several root abnormalities and changes in their flowering cycle, with kinetin inhibiting flowering in tomatoes, but accelerating it in peas.

You can see in this study that the effective concentration is quite low. The range of kinetin concentrations tested goes from 0.0215mg/L to 2.15 mg/L. These values are quite small compared to the amounts of other hormones, such as IBA or NAA, generally used in plant culture. The concentration of kinetin plays a key role in its effect. A 2008 study on red goosefoot (3) shows the strong impact kinetin concentration can have. These researchers showed that low concentrations of kinetin increased bud formation and increased the height of the apical meristem, while large concentrations inhibited flowering and made the plants shorter.

The entire literature on exogenous kinetin applications is therefore split between apparently contradictory effects. Some studies show effects that are more in line with a gibberellin inhibitor, with shorter plants, while others show stimulation of shoot growth. What you get is dependent on concentration and plant species, making kinetin a hard hormone to use. Use too much and you might compromise flowering and yields, use too little and you might have undesirable elongation effects or simply no effects at all (4, 6).

Kinetin can also have an effect on the sex determination of plants. For example, kinetin induces female flowers in cannabis and can ameliorate the production of male flowers in female plants (12).

Kinetin foliar sprays

The mode of application makes a big difference as well. While most of the root studies I read using kinetin kept their application rates below 3mg/L, many foliar studies explore kinetin application rates that are significantly higher. In this study (9), for example, they perform kinetin applications at 100 ppm. From the foliar studies I read, I found this study (7) particularly interesting. In it, kinetin applications at 2.5, 5, and 10 mg/L were done using foliar spraying on tomato, cucumber, and pepper plants.

The researchers found that the cucumbers had an excellent response to the 2.5 mg/L treatment, with taller plants, larger leaf area, and bigger yields, while they showed negative responses to the 10ppm treatment, with lower yields. While tomatoes showed a similar response, peppers gave their best results with the 10 ppm kinetin sprays. This again highlights not only that plants will respond negatively to excessive doses of kinetin, but that this response is significantly species-dependent.

Environmental conditions

Furthermore, environmental conditions can play a significant role in the effects of kinetin. This study (8) found that kinetin could help rice plants give better yields under carbon dioxide enrichment. However, this worked only for some of the varieties of rice used. For the varieties for which it worked, kinetin applied as a foliar at 10.75 ppm was able to enhance the carbon dioxide fertilization effect.

Other environmental conditions, such as salinity stress and oxidative stress, can also play a big role in the effect of kinetin. As a strong antioxidant, kinetin can help plants deal with oxidative stress (10). It has also been tested many times as a way to deal with salinity-induced stress, for example, see this article on kinetin applications in soybeans (11). In this last study, you can see how kinetin upregulates the gibberellin biosynthesis pathway when it was actively suppressed by the high salinity. Some effects, such as the production of jasmonic acid, are actually opposite in the control and in the salinity-induced environments as a function of kinetin concentration.

Conclusion

Kinetin can be a powerful and versatile hormone in flowering plants. It can be used to achieve a variety of different effects, including making plants shorter, increasing budding sites, increasing yields, or relieving sources of stress. However, the choice of concentration, method, and application time is critical and can lead to completely opposite effects if not done correctly. Low applications tend to increase growth and leaf area, while larger concentrations will show an effect similar to a gibberellin inhibitor. However, the concentrations that work best for a given plant cannot be known before experimentation is done. However, do consider that higher concentrations consistently lead to decreases in yields.

If you want to use kinetin in your crop, start with a foliar dose at around 2ppm and take note of the effects. From there, you will be able to gauge whether you want to have a higher or lower concentration of kinetin. If the dose is too high, you will start to see some negative effects. Also, time your applications so that they are in line with the effects you want to achieve. If you want to feed kinetin through the roots, use an even lower concentration and make sure your applications are properly timed, avoid having permanent exposure of roots to kinetin, as this is likely to be negative.

Have you ever used kinetin in your crops? What concentrations have you used and what effects have you seen? Let us know in the comments below!

Fulvic and humic acids have been studied for decades and used extensively in the soil and hydroponic growing industries. I previously talked about the use of humic acid in hydroponics and the way in which it can improve crop results. In that post, we talked about how humic acids can improve nutrient chelation and how this can lead to improvements in yields depending on the origin and properties of the humic substances used. In this post, we are going to take a look specifically at fulvic acid substances, which are a smaller family that has potentially more valuable uses in the hydroponic space. We will start by discussing what differentiates fulvic and humic acids and what the current peer-reviewed evidence around fulvic acids tells us.

Fulvic acids are not chemically pure substances, but a group of chemicals that result from the decomposition of organic matter. This process generates both humic and fulvic acids. However, fulvic acids are different from humic acids in mainly two ways. The first is that fulvic acids are soluble at both acid and alkaline pH values, and the second, is that fulvic acids generally have much lower molecular weights. Fulvic acids are therefore more soluble and are more easily accessible to plants compared to humic acids, which have much larger molecular weights. But why should we use them in hydroponics and exactly how?

Sadly, not many publications have tackled the use of fulvic acid in crops specifically. One of the few examples of reviews that touch on the matter is this paper, which covers most of the literature around fulvic acids before 2014. I also did a literature review myself, trying to find articles in which the fulvic acid source, application type and rate, and the results against a control without fulvic acid were clearly explained. The table below shows you the results of my search, I was able to find 10 papers overall, with a mix of root and foliar applications of fulvic acid, with a range of application rates and plant species. Almost all of these papers found positive results from the use of fulvic acid, except two papers that found either no effect or mixed results from their use.

The range of application depends substantially on the application type. Most papers that tackled foliar applications chose application rates in the 1-3g/L range, while papers that tackled root applications generally stayed in the 25-150ppm range. This is normal since foliars are generally much more concentrated than root applications. Both types of applications have different effects. Root applications are going to exercise an additional strong nutrient chelating role, while foliar applications are more likely to exert a hormonal role. A study around genetically modified tomato plants showed that plants engineered to be insensitive to IAA were also unable to respond to fulvic acid, hinting at the fact that fulvic acid has an auxin-like effect in plants.

The effects seem to be quite positive overall, with increases in yield, quality, and flower numbers across the board. The studies above that investigated nutrient transport also showed substantial benefits when root applications of fulvic acid were used. Plants grown in a Hoagland solution showed better nutrient transport when fulvic acid substances were used in the nutrient solution. This is possibly both due to their ability to chelate micronutrients and their ability to provide an additional pH buffer at the region of interest in hydroponics (5.5-6.5). This study, shows how fulvic acid substances can have pKa values in this precise region, although their still relatively large molar mass implies that they will contribute marginally to buffering capacity, especially if used only in <100 ppm concentrations.

Fulvic acids also seem to be synergistic with several other biostimulants in the studies showed above. When tests were done with humic acids or other biostimulants, the effect of the combination is usually better than the effect of either part on its own. This means the fulvic acid might not only be a good addition on its own, but it might also contribute significantly to enhance the effect of other biostimulants used.

It is however important to note that fulvic acids do have negative effects when used in excess, reason why their application rates need to be carefully controlled. Using too much can lead to drops in yields and quality along with slower growth. If you want to start using them, it is, therefore, wise to start at the lower range of the application rates shown above and climb up as you gauge the effects. It is also important to note that – as humic acids – different sources of fulvic acid might have different effects, as the actual molecules that make up the substance will change.

A big advantage of the use of fulvic acids in hydroponics is also that their solubility is quite high, so the risk of clogging or damaging equipment is low. This is a significant advantage over humic acids, which have lower solubility and can cause problems because of this in hydroponics culture, especially if there are drops in the pH. In hydroponics, fulvic acids can also lead to additional solution stability, especially in recirculating systems, where the destruction of heavy metal chelates as a function of time can become a bigger risk.

All in all, fulvic acids represent a relatively cheap addition to a hydroponic regime that has limited risk and a lot of potential upsides. Literature research shows us that low rate applications, if anything, might just have no effect, so the risk of damage to a hydroponic crop by trying fulvic acid applications is low. The synergistic effects shown by fulvic acid are also interesting since this means that they might make other additives you are currently using even more potent. When looking for fulvic acids, make sure you check for high solubility, solubility in low and high pH, and a source that matches the sources used in the literature results you’re interested in reproducing.

Few bio-stimulants are more popularly used than seaweed/kelp extracts. These are used by many growers to increase plant quality and yields, in particular, extracts from the Ascophyllum nodosum species are an all-time favorite of the industry. These extract have also been studied extensively for the past 40 years, with large amounts of evidence gathered about their effects and properties across several different plant species. In this article, I will be talking about what the research says about their use, why these extracts work, how these have usually been applied and what you should be looking for when using this type of bio-stimulant.

The use of kelp extracts is so common, that there was already enough research done about their use to publish a review on the subject in 1991 (1), a lot of the information below comes from this source. Seaweed has been used by farmers for hundreds of years, as it could be used as an alternative to lime in order to alkalinize acidic peatmoss soils, due to the high basicity of seaweed extracts (as some are very high in calcium carbonate content). Seaweed extracts also contain a lot of micro and macro nutrients – as shown above – in proportions that are useful for their use as fertilizer. They are a significant source of potassium and calcium, although the variability of the composition – as shown in the table above – can be quite important. They also contain micronutrients but their low presence relative to plant needs implies that the positive effects of the extracts are most likely not due to them.

Perhaps one of the most important factors surrounding seaweeds is their content of bioactive molecules. These extracts contain an important array of cytokinins, which are plant hormones that will significantly affect plant growth. Auxins, gibberillin-like substances and ethylene precursors like aminocyclopropanecarboxylic acid, have also been detected in seaweed extracts. The cytokinins are usually present in concentrations of around 2-20 ppm in the concentrated extracts, which are enough to cause effects, even if the final diluted versions will be at much lower concentrations. The application of seaweed extracts is usually done through an entire crop cycle and is usually cumulative in nature.

Application rate, frequency, seaweed species and extract processing methods can substantially affect results, with many contradictory results showing up in the literature, with some people showing increases in growth and yields while others show no effects at all. The review quoted above describes many examples of positive results, including examples showing weight gains, yield gains and increases in certain nutrients, like P and N. The review also talks about the ability of seaweed extracts to increase resistance to pests and improve crop quality. A more recent review from 2014 (2) further expands on a lot of these positive effects, citing extensive literature showing increases in yields, dry weights and quality for a wide variety of plant species. In total, more than 30 different papers showing increases in yields due to the use of kelp extracts are cited in this review. There are also more than 20 articles cited describing increases in disease resistance or other mechanisms of defense elicitation due to the use of the seaweed extracts.

Foliar applications of seaweed can be carried out at varied levels of frequency and concentration. Applications at a 0.2-0.5% w/v of dry extracts are most common, although higher or lower concentrations have also been found to be effective. As a root drench applications will tend to be on the lower side, as the seaweed contains a substantial amount of NaCl, which can be damaging to plants. Timing of applications can also be quite critical, some growers apply the extract equally spaced through the entire growing periods, while others attempt to time the application with a specific growth phase. Success is reported in both cases, although papers that describe different timing of single applications often find significant differences. To arrive at the optimal usage for a plant species it will be necessary to carry out tests with single applications at different intervals, although single weekly applications are likely to be successful if a less involved approach is desired.

Although the use of seaweed extracts can be very positive, it is also worth mentioning that it is very dependent on the quality and consistency of the extract being produced. Since we know that most of the positive effects of these seaweeds are related to their plant hormone content, their use can sometimes be replaced with specific applications of plant hormones, if the effects are properly understood. The discussion in (2) cited before points to the fact that kinetin applications have been able to match the effects of kelp extracts, at a fraction of the cost and the environmental impact at least in a few cases.

With all the above said, it is quite evident that kelp/seaweed extracts have been widely confirmed to have positive effects in the growing of plants, beyond any reasonable doubt. This effect is mostly related with the hormones they contain and is therefore dependent on the seaweed species, where it is grown and how the seaweed powder is generated. Although root and foliar applications of kelp can both be used to improve results, the use of foliar applications is often favored in order to avoid the introduction of some undesired ions into the growing media. If you’re not using kelp, go ahead, it’s bound to help!

Plants normally get most or all of their phosphorous from inorganic phosphorus sources. Most commonly these sources are monobasic or dibasic phosphate ions (H₂PO₄^– and HPO₄^-2), which are naturally formed from any other phosphate species at the pH values generally used in hydroponics (5.5-6.5). However these are not the only sources of inorganic phosphorous that exist. Phosphite ions – which come from phosphorous acid H₃PO₃ – can also be used in plant culture. Today we are going to talk about what phosphite does when used in hydroponics and why it behaves so differently when compared with regular phosphate sources. In research P from phosphate is generally called Pi, so I will follow this same convention through the rest of this post. A good review on this entire subject can be found here.

The role that phosphite (Phi) plays in plant nutrition and development has now been well established. Initially several people claimed that Phi was a better P fertilizer than Pi so researchers wanted to look into this to see if Phi could actually be used as an improvement over Pi fertilization. However research was heavily disappointing, studies on lettuce (here) , spinach (here), komatsuna (here) as well as several other plants showed that Phi fertilization provides absolutely no value in terms of P nutrition, meaning that although plants do absorb and process the Phi it does not end up being used in plant tissue to supplement or cover P deficiency in any way. Furthermore there are some negative effects when Phi is used in larger concentrations (as those required for Pi) so it quickly became clear that Phi is not a good fertilizer at all.

Why should anyone use Phi then? Well, research started to show that some of the earlier positive results of Phi fertilization were not because it was covering Pi deficiencies but mainly because it was offering a protective effect against some pathogens. Research on tomatoes and peppers and other plants (here and here) showed that phosphites had some ability to protect plants against fungi with plants subjected to Phi applications showing less vulnerability to the pathogens. However the evidence about this is also not terribly strong and a few papers have contested these claims.

Those who say that Phi is not mainly a fungicide claim that positive results are mainly the effect of Phi acting as a biostimulant (here). These groups have shown through research across several different plant species, including potatoes, onions, lettuce, tomatoes, wheat, oilseed rape, sugar beet and ryegrass that foliar or sometimes root applications of phosphites consistently yield some positive effects, meaning that there is a strong biostimulant effect from the Phi that is not related to either P nutrition or a fungicidal effect. A recent review looking at the overall biostimulant effects of Phi (here) shows how researchers have obtained evidence of biostimulation in potatoes, sweet peppers, tomatoes and several other species (the images in this post were taken from this review). The different studies mentioned in the review show increases in quality and even yields across these different plant species (see tables above).

While we know that Phi is not a good source of P nutrition and we know it can help as a fungicide in some cases it is clear now that under enough Pi nutrition Phi can provide some important biostimulating effects. Negative effects from Phi seem to be eliminated when enough Pi nutrition is present so rather than be thought of as a way to replace or supplement P nutrition it should be thought of as an additive that has a biostimulating effect. Phi may become a powerful new tool in the search for higher yields and higher quality, while not serving as a replacement for traditional Pi fertilization.

Plants and microorganisms affect the substrates in which they grow in many ways. If you start growing plants in an inert substrate – with nutrient applications of course – you will notice that the substrate’s chemical composition will start to change with time and it will start to get enriched in carbon containing substances. As plants and microorganisms grow, thrive and die, some of the chemicals that made up their cells end up enriching the substrate they grow on. This process – whereby organic materials from living organisms become part of a substrate – is what generates the soils around us. One of the most prevalent class of components in this organic material, is what we call humic acids.

Humic acid is not a single substance but a wide range of substances that are created as a product of plant and microorganism decomposition. This is why you often hear people talk about “humic acids” instead of simply “humic acid”. They are called “acids” because the humic substances contain molecules that have groups that resemble those found in phenol and vinegar. They are also differentiated from fulvic acids in the fact that they are only soluble at basic pH values while fulvic acids are generally small enough molecules to be soluble across most of the pH spectrum. Since humic acids are a very important component of enriched soils and can be used in soiless culture, people have started using them as supplements in soiless and pure hydroponic culture.

When talking about the effects of humic acids it is worth mentioning that since we’re talking about a group of molecules – not a single substance – effects are generally dependent on the source of the humic acid used. For example you can find a study on tomatoes here where two different sources of humic acids – from peat and leonardite – were used to grow tomatoes. The study shows a clear difference between both with the first only stimulating root growth while the second stimulated both roots and shoots. However in both cases there was an increased iron availability to plants, although the mechanism for this was not established.

In plants like gerberas humic acids applied at 1000 ppm can offer increases in harvested flowers of up to 52% (see here), somewhat positive effects can also be seen in tomatoes across the literature with most studies showing increases in yields and mineral contents (see here), reports of positive effects on gladiolus have also been published (here). Since the 1990s there has been a somewhat established understanding of some general beneficial effects for humic acid applications, it is well established that they can prevent and eliminate micro nutrient deficiencies due to their abilities to increase their availability(see here). The literature is also quite consistent in that the largest effects are often seen on root growth rather than on shoot growth or mass. There are however some types of humic acids that have showed higher increases of shoot mass, for example in an article studying humic substances derived from municipal waste on barley this was the observed effect. For some plants however – despite these beneficial effects – increases in yields in hydroponic culture are not evident (see here and here). A look at the effect of a humic acid source on several different plant species can be found here.

It is worth noting that humic acid applications are also not limited to the root zone. Since humic acids can enhance the absorption of some nutrients they can also be applied in foliar sprays. Experiments on strawberries (here) showed that an application of 1.5-3ppm of humic acids led to an increase in the quantitative and qualitative properties of the fruits.  Combinations of humic acids with other biostimulants are also common. For example a combinations of lactate, humate and beneficial bacteria was tested on tomatoes (here) but the experiments showed that the effect could be stimulating or inhibiting depending on the particular conditions, even though most combinations were beneficial.

With the high variability between humic substance origins, application rates and effects it is very hard to say whether humic acid applications will definitely help your crops in terms of yields. For almost all humic acid sources it is probably warranted that micronutrient absorption will be somewhat augmented due to their ability to chelate these nutrients, but only if the nutrients are not efficiently chelated already. This sole ability might lead to crop improvements if deficiencies are present but improvements in yields will strongly depend on humic acid substance origin and particular properties. However humic acids do seem to lead to general product quality improvements and since negative effects are rare there seems to be no harm in carrying out field tests to determine if their use is worth it for your particular crop.

Usually additives used in hydroponics need to be added in rather large quantities to obtain palpable results. Molecules like salicylic acid – which we have discussed before – need to be used in concentrations in the order of 10^-4 to 10^-2 M to obtain a significant effect. This means that you need to use quantities in the order of 20-150ppm of most additives in order to see a significant result. However there is a molecule called 1-triacontanol that can generate very significant results with only a fraction of that concentration. Today we will talk about this substance, what it does, how to use it and why it’s such a desirable tool in your hydroponic additive arsenal. Many of the things I will talk about in this article are derived from this 2011 review on triacontanol (make sure you read that for a deeper insight into why this molecule works).

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Triacontanol is a very long fatty alcohol. Each molecule has 30 carbon atoms linked in a linear structure which makes this molecule extremely hydrophobic and hence very hard to dissolve in something like water. Using triacontanol therefore involves dissolving this molecule in something other than water –  for example Tween 20, chloroform, methanol – before adding water in order to prepare an emulsion for use in either root applications or foliar feeding. Most research using triacontanol has used foliar feeding as this is the easiest way to control the application of the molecule and also how it seems to have the largest effect.

The effects of this molecule are not short of miraculous. Triacontanol is usually applied in concentrations on the order of 10^-7 to 10^-9 M, which means it is used from around 0.01 to 1 ppm. This means that we use about 1000 times less triacontanol than other additives in order to obtain a meaningful result. The table below shows some of the effects that triacontanol has showed in peer reviewed studies, with plant height, weight and yields increasing across a variety of different species, from tomatoes to japanese mint. Papers on other plants besides those on the chart have also been published, for example triacontanol has showed to significantly increase yields in lettuce crops (here).  Some studies have also found that the effect of triacontanol can also be enhanced through the use of magnesium or in conjunction with other hormones (here).

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With such an impressive array of effects and such a low expected toxicity – due to its very low solubility – it’s definitely one of the best additives to use to get production gains in hydroponic crops. This also makes it one of the most commonly used substances in commercially available grow enhancers. Nonetheless since it’s used in such a small quantity it’s very easy for someone to buy a small amount of triacontanol and use it for years before running out. You can buy small amounts of triacontanol as a powder (there are several reputable sellers on ebay) and you can then prepare your own concentrated triacontanol solution in Tween 20 – not water – that will last you for ages. A liter of 2000ppm solution of triacontanol will last you for 1000-2000 liters of foliar spray. You cannot get more economical than that.

The optimum application rate and frequency for triacontanol varies across different species but if you want to take an initial guess use a foliar application of a 0.5 ppm solution every week. There is usually a sweet spot for concentration – after that you start to see a decrease in results compared to the highest point – so you want to start below a 1 ppm application rate. For some crops repeated applications might be unnecessary – with just one or two applications giving most of the effect through the entire crop cycle – while for others you do want to apply every week. How you initially dissolve the triacontanol to make your concentrated solution is also important with Tween 20 being the most ecologically friendly – although not the easiest – option.

When looking for ways to increase crop yields we usually want something that is safe for the environment, safe for us and able to give us a substantial bang for our buck. From the multitude of additives that have been researched during the past 30 years one simple organic molecule seems to fit all the requirements very well: salicylic acid. Today we are going to talk about why this additive is so interesting for use in hydroponic culture, the results it has shown in peer reviewed publications and how we can use it to increase our crop yields. For those of you interested in this molecule I would also recommend reading this 2010 review which contains a much more detailed look into the scientific literature surrounding salicylic acid research in higher plants.

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Salicylic acid is a simple organic molecule with the structure showed above. We have known for a long time that plants produce it and we knew almost right from the start that it played a key role in plants’ response to diseases and stress (see here for some early insights from Tobacco cultivation). Salicylic acid is used as a signaling molecule in plants (a.k.a hormone), moving from stressed organs to non-stressed ones as the plant is attacked. However its role is much more complex, having functions related with chloroplast creation, inhibition of fruit ripening and many other important processes.

After learning that this was an interesting molecule it wasn’t long before people started studying whether exogenous applications provided any benefit. We have learned that it enhances dry mass and leaf area in corn and soybean (here), that it can enhance germination in wheat (here), the oil content in basil (here), the carbohydrate content in maize, etc. There are also several studies pointing to improvement in root development – even from foliar applications (here) – suggesting that this hormone is able to increase plant productivity through several different mechanisms. The incidence of diseases can also be reduced dramatically by salicylic acid applications (here).

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We also know this molecule has important effects on the flowering process. It can induce earlier flowering in plants and can often cause larger fruit settings in some plants (like papaya (here)). Most importantly foliar spraying of tomato and cucumber plants with salicylic acid has showed important increases in yields (here). It is therefore clear that exogenous applications of salicylic acid can have many important benefits in crop production and this is therefore an important candidate to consider for enhancing crop production.

But how do we apply it? Most commonly this molecule is applied in foliar feeding regimes, although in some cases it is also applied directly in hydroponic solutions. Most commonly concentrations in the order of 10^-5 -10^-4 M are used since it has been showed across a few studies that negative effects start to show up when the concentration level reaches 1mM. This means that regular doses will be around 1-100 ppm with the lowest spectrum of dosage being preferred if the effect on the particular plant is unknown. The solubility of salicylic acid is 2.48g/L at 25°C so concentrated solutions of up to around 20-30x can be prepared without issues to make it easier to apply on plants. The preparation of more concentrated solutions requires some tricks but it certainly can be done.

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Salicylic acid also has the advantage of being a very safe molecule so it can be applied without a lot of worry in order to experiment with its effects. For testing on new plants foliar applications of 20-30ppm would be most common, with applications usually carried out once every 5-10 days. The frequency of application as well as the best concentration to use will of course depend on the particular plant you’re growing. There are also several other molecules that can be used with salicylic acid to enhance its effect on some plants, but this will be the focus of a future post.

Finally it is also worth noting that salicylic acid is not aspirin (aspirin is acetylsalicylic acid, a related yet different molecule) so if you want to experiment with this additive you should buy salicylic acid instead of just “dumping some aspirin” into your foliar or hydroponic nutrient solution.

There are many additives that can be used to enhance the yield of flowering crops. Some have been covered in this blog – like silicon – while others haven’t been mentioned here. Today we are going to talk about a rarely discussed additive that is infrequently used in plant culture these days: Titanium. I want to talk about this additive in light of a literature review that came up recently (April 2017) about the use of Titanium in crop production. The magazine where this review came from (Frontiers in Plant Science) is a magazine that often has good content in the field of innovative crop enhancing techniques.

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Titanium use in plant culture is not new. From the early 1980s people started to experiment with titanium as techniques were developed in order to produce titanium chelates that could be used in foliar applications. Basically all reports of yield increases – that show wonderful increases up to even 95.3% in yields – come from a paper on the biological importance of titanium by Dr. István Pais in 1983 and then another publication in 1991 by the same person (here). Other authors have also showed increased yields (here and here) although in some cases in conjunction with other additives (like Si) with results often much less dramatic than the initial 1983 papers. Titanium nanoparticles have also been tested and their effect has mostly been negative with decreases in plant growth and often DNA damage. For this reason when using titanium you want to go with a soluble chelate and not nanoparticle sources.

Creating aqueous stable Ti is not a cake walk. There is currently only one product that carries water soluble Ti (called Tytanit) and as far as I can tell no other commercial products for the application of Ti exist at this moment. This tytanit product is most probably titanium ascorbate – the most popular chelate used – but other organic chelates, like Ti citrate, might be usable as well. Preparing Ti ascorbate is not so easy to get as well – you cannot just buy it on ebay/alibaba as it’s not stable as a solid – so you need to prepare it from scratch. Titanium chemistry in solution is sadly very complicated.

However there is probably a route to the easy preparation of such complexes using a simple method involving titanium dioxide and ascorbic acid. We know from dissolution studies of titanium dioxide that it can be dissolved significantly by ascorbic acid but the final concentration of these solutions is not very high with a final concentration of around 0.025M of Ti possible in solution using this method, with a surrounding concentration of 0.15M of ascorbic acid. More acid does not help dissolve more titanium dioxide as this seems to be the solubility limit of the titanium complex. This gives you around 1.2g/L of Ti which you need to dissolve 500-1000x to arrive at the recommended application rate of 1-2 ppm. This will give a final ascorbic acid concentration of 26ppm which is acceptable as an additive as well.

Obviously there are some further formulation steps necessary to get the above to work correctly but this outlines the basics to develop a concentrated titanium ascorbate product that can be used for the creation of a Titanium supplement. Industrally this can be achieved much more efficiently with the use of titanyl sulfate which is a readily soluble and easy to get industrially – but hard to get for your home – form of titanium. You can see this patent for examples of how a fertilizer using titanyl sulfate can be prepared.

Evidence about titanium – applied as titanium ascorbate in a foliar spray – being positive for crops is significant. Various positive effects have been shown across a significant variety of plants across several different plant types – tomatoes, beans, peppers – by different authors. The effect on yields is not so clear – probably in reality not as large as shown in the original studies, but probably significant enough to warrant further studying. The development of low-cost processes for the manufacturing of titanium fertilizers will further enhance their use and increase our knowledge about their true capabilities. More studies with ascorbic/ascorbate controls will also show us clear evidence of whether we are seeing effects related with the ascorbate or the actual Ti chelate.